Journal: Medical Journal of the Islamic Republic of Iran

Article Title: Human papilloma virus: A review study of epidemiology, carcinogenesis, diagnostic methods, and treatment of all HPV-related cancers

doi: 10.47176/mjiri.35.65

Figure Lengend Snippet: The classification of commercially available diagnostic techniques for the detection and genotyping of HPV

Article Snippet: , , PCR-based fluorescent bead array , ❖ Microarray-based HPV genotyping assays ○ The PapilloCheck HPV Screening Test(greiner bio), ○ Clart HPV 2 – papillomavirus clinical arrays( Genomica), ○ HPV GenoArray Test Kit ( Hybribio), ○ GeneTrack HPV DNA Chip( Daejeon), ○ GeneSQUARE HPV Microarray,( Kurabo) ○ INFINITI HPV-HR QUAD (AutoGenomics), ○ PANArray HPV Genotyping Chip( Panagene), ○ GG HPVCHIP ( Goodgene) ❖ Suspension array based HPV genotyping assays ○ The Multiplex HPV Genotyping Kit (Multimetrix, Heidelberg) ○ digene HPV Genotyping LQ Test(Qiagen).

Techniques: Diagnostic Assay, Amplification, Nested PCR, Multiplex PCR, Hybridization, Microarray, Suspension, Multiplex Assay, Real-time Polymerase Chain Reaction, HPV Assay, Southern Blot, In Situ Hybridization, Neutralization

Journal: Medical Journal of the Islamic Republic of Iran

Article Title: Human papilloma virus: A review study of epidemiology, carcinogenesis, diagnostic methods, and treatment of all HPV-related cancers

doi: 10.47176/mjiri.35.65

Figure Lengend Snippet: The classification of commercially available diagnostic techniques for the detection and genotyping of HPV

Article Snippet: , , PCR-based fluorescent bead array , ❖ Microarray-based HPV genotyping assays ○ The PapilloCheck HPV Screening Test(greiner bio), ○ Clart HPV 2 – papillomavirus clinical arrays( Genomica), ○ HPV GenoArray Test Kit ( Hybribio), ○ GeneTrack HPV DNA Chip( Daejeon), ○ GeneSQUARE HPV Microarray,( Kurabo) ○ INFINITI HPV-HR QUAD (AutoGenomics), ○ PANArray HPV Genotyping Chip( Panagene), ○ GG HPVCHIP ( Goodgene) ❖ Suspension array based HPV genotyping assays ○ The Multiplex HPV Genotyping Kit (Multimetrix, Heidelberg) ○ digene HPV Genotyping LQ Test(Qiagen).

Techniques: Diagnostic Assay, Amplification, Nested PCR, Multiplex PCR, Hybridization, Microarray, Suspension, Multiplex Assay, Real-time Polymerase Chain Reaction, HPV Assay, Southern Blot, In Situ Hybridization, Neutralization

Journal: Medical Journal of the Islamic Republic of Iran

Article Title: Human papilloma virus: A review study of epidemiology, carcinogenesis, diagnostic methods, and treatment of all HPV-related cancers

doi: 10.47176/mjiri.35.65

Figure Lengend Snippet: The classification of commercially available diagnostic techniques for the detection and genotyping of HPV

Article Snippet: , , PCR-based fluorescent bead array , ❖ Microarray-based HPV genotyping assays ○ The PapilloCheck HPV Screening Test(greiner bio), ○ Clart HPV 2 – papillomavirus clinical arrays( Genomica), ○ HPV GenoArray Test Kit ( Hybribio), ○ GeneTrack HPV DNA Chip( Daejeon), ○ GeneSQUARE HPV Microarray,( Kurabo) ○ INFINITI HPV-HR QUAD (AutoGenomics), ○ PANArray HPV Genotyping Chip( Panagene), ○ GG HPVCHIP ( Goodgene) ❖ Suspension array based HPV genotyping assays ○ The Multiplex HPV Genotyping Kit (Multimetrix, Heidelberg) ○ digene HPV Genotyping LQ Test(Qiagen).

Techniques: Diagnostic Assay, Amplification, Nested PCR, Multiplex PCR, Hybridization, Microarray, Suspension, Multiplex Assay, Real-time Polymerase Chain Reaction, HPV Assay, Southern Blot, In Situ Hybridization, Neutralization

Journal: Medical Journal of the Islamic Republic of Iran

Article Title: Human papilloma virus: A review study of epidemiology, carcinogenesis, diagnostic methods, and treatment of all HPV-related cancers

doi: 10.47176/mjiri.35.65

Figure Lengend Snippet: The classification of commercially available diagnostic techniques for the detection and genotyping of HPV

Article Snippet: , , PCR-based fluorescent bead array , ❖ Microarray-based HPV genotyping assays ○ The PapilloCheck HPV Screening Test(greiner bio), ○ Clart HPV 2 – papillomavirus clinical arrays( Genomica), ○ HPV GenoArray Test Kit ( Hybribio), ○ GeneTrack HPV DNA Chip( Daejeon), ○ GeneSQUARE HPV Microarray,( Kurabo) ○ INFINITI HPV-HR QUAD (AutoGenomics), ○ PANArray HPV Genotyping Chip( Panagene), ○ GG HPVCHIP ( Goodgene) ❖ Suspension array based HPV genotyping assays ○ The Multiplex HPV Genotyping Kit (Multimetrix, Heidelberg) ○ digene HPV Genotyping LQ Test(Qiagen).

Techniques: Diagnostic Assay, Amplification, Nested PCR, Multiplex PCR, Hybridization, Microarray, Suspension, Multiplex Assay, Real-time Polymerase Chain Reaction, HPV Assay, Southern Blot, In Situ Hybridization, Neutralization

Journal: Medical Journal of the Islamic Republic of Iran

Article Title: Human papilloma virus: A review study of epidemiology, carcinogenesis, diagnostic methods, and treatment of all HPV-related cancers

doi: 10.47176/mjiri.35.65

Figure Lengend Snippet: The classification of commercially available diagnostic techniques for the detection and genotyping of HPV

Article Snippet: , , PCR-based fluorescent bead array , ❖ Microarray-based HPV genotyping assays ○ The PapilloCheck HPV Screening Test(greiner bio), ○ Clart HPV 2 – papillomavirus clinical arrays( Genomica), ○ HPV GenoArray Test Kit ( Hybribio), ○ GeneTrack HPV DNA Chip( Daejeon), ○ GeneSQUARE HPV Microarray,( Kurabo) ○ INFINITI HPV-HR QUAD (AutoGenomics), ○ PANArray HPV Genotyping Chip( Panagene), ○ GG HPVCHIP ( Goodgene) ❖ Suspension array based HPV genotyping assays ○ The Multiplex HPV Genotyping Kit (Multimetrix, Heidelberg) ○ digene HPV Genotyping LQ Test(Qiagen).

Techniques: Diagnostic Assay, Amplification, Nested PCR, Multiplex PCR, Hybridization, Microarray, Suspension, Multiplex Assay, Real-time Polymerase Chain Reaction, HPV Assay, Southern Blot, In Situ Hybridization, Neutralization

Journal: eLife

Article Title: Association with TFIIIC limits MYCN localisation in hubs of active promoters and chromatin accumulation of non-phosphorylated RNA polymerase II

doi: 10.7554/eLife.94407

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , LT3GEPIR , Addgene , Cat# 111177 Zuber , Tet-ON miR-E (miR-30 variant)-based RNAi.

Techniques: Recombinant, Expressing, Plasmid Preparation, Variant Assay, Sequencing, shRNA, ChIP-qPCR, In Situ, Affinity Purification, Fluorescence, Isolation, Multiplex Assay, Transfection, Magnetic Beads, Software, Imaging

Journal: Blood

Article Title: Rapid single-molecule digital detection of protein biomarkers for continuous monitoring of systemic immune disorders

doi: 10.1182/blood.2019004399

Figure Lengend Snippet: PEdELISA assay platform for monitoring CAR T-therapy associated CRS. (A) Concept of the instantaneous single-molecule binary counting of pre-equilibrium protein-binding events. The combination of pre-equilibrium reaction quenching with single-molecule counting can theoretically achieve an assay with a near-0 incubation time without losing linearity. (B) Schematic and photo image of the PEdELISA system, which comprises a disposable microfluidic chip (inset), an automated fluidic dispensing and mixing module (left), and a 2-dimensional inverted fluorescence scanning module (right). (C) Two-step ultrafast, multiplex PEdELISA process for the pre-equilibrated assay system, including 5-minute magnetic bead incubation for the formation of antibody-antigen-antibody immune-complexes (step 1), buffer exchange, 1-minute avidin-HRP labeling (step 2), and 5-minute continuous washing using the automated fluidic dispensing module. The PEdELISA chip has 8 circular biosensor patterns formed by a cluster of 66 724 arrayed microwells. Fluorescence color-encoded magnetic beads coated with different capture antibodies (nonfluorescent and Alexa Fluor 488 [AF 488]) are pre-deposited into each physically separated biosensor pattern. This arrangement can permit multiplex analyte detection with 2 colors × 8 patterns = 16 plexes. Each chip can quantify up to 16 samples simultaneously per batch run. (D) Digital readout process, which involves loading of HRP fluorescence substrate (QuantaRed), sealing of beads with fluorocarbon oil, and signal reading based on automated fluorescence scanning to count fluorescently activated “on”-state microwells. Data analysis is then performed by a convolutional neural network–guided image-processing algorithm for high throughput and accurate single-molecule counting.

Article Snippet: Each chip can quantify up to 16 samples simultaneously per batch run. (D) Digital readout process, which involves loading of HRP fluorescence substrate (QuantaRed), sealing of beads with fluorocarbon oil, and signal reading based on automated fluorescence scanning to count fluorescently activated “on”-state microwells.

Techniques: Protein Binding, Single Molecule Counting, Incubation, Fluorescence, Multiplex Assay, Buffer Exchange, Avidin-Biotin Assay, Labeling, Magnetic Beads, High Throughput Screening Assay